; Grishchenko, A.E. Ionic Polymers Based on Dextran: Hydrodynamic Properties in Aqueous Solution and Solvent Mixtures. The microporation device based on radiofrequency can be used to enhance delivery efficiency by removing the epidermis layer. The Hydrodynamic Radii of Macromolecules and Their These results are in agreement with a previous study which suggested that the microporation using radiofrequency causes disruption of the SC, enabling the percutaneous permeation of the FITC-dextran [21]. dextran Format). Journal of American Chemical Society, 76 (20), 5041-5052. Hindered diffusion of dextran and ficoll in microporous membranes. Harding, Stephen E. (1997). where tr is the column retention time in minutes (2 of 0.9792). (2008). Proc of SPIE. 2005;22(4):5505. Pathan IB, Setty CM. As intrinsic viscosity of dextrans decreases with an increase in solution temperature; chain flexibility enhances with an increase in temperature (see Table 4) showing that the intrinsic viscosity of dextran (with different molecular weights Mw from 8.8 to 200kDa) decreases linearly with an temperature increase in solution, but change to high molecular weight to hyperbranched macromolecule. To improve drug permeability, micropore ablation using RF is performed by passing an alternating electrical current through the skin at a frequency higher than 100kHz. The regeneration process of the skin is regulated by growth factors such as keratinocyte growth factor and fibroblast growth factor, which trigger the proliferation and migration of cells [23, 24]. Analytes and stationary phase with Universal Calibration Relative Measurement Concentration and MW detector: RI + Viscometer Relationship between hydrodynamic volume (MW*intrinsic viscosity) and elution volume Multiple detection The skin culture medium was purchased from Biopredic International (Saint-Grgoire, France). The relation between Mw and the intrinsic viscosity is given by Mark-Houwink equation. Microporations were performed 2, 5, and 10 times and the skin damage was confirmed using the TEWL measurement. Determination of constants, kM-H and a, from the intrinsic viscosity data, requires a series of monodisperse polymer samples with known molecular weight or a series of polydisperse polymer samples with known viscosity average molecular weights, Mv. Table 1 shows some values of dextrans Mark-Houwink parameters, from literature consulted, where all data are based on the logarithmic plot of intrinsic viscosity [] and molecular weight Mw. Biophysical Journal, 87, 4259-4270. 2021 Aug;13(4):915-925. doi: 10.1007/s12602-021-09751-1. Polymer molecular weight distribution. official website and that any information you provide is encrypted Masuelli, Martin A. Hydrodynamic properties of whole arabic gum. These results indicate that healing and regeneration occurred in the human cadaver skin after the microporation. Fusco V, Quero GM, Cho GS, Kabisch J, Meske D, Neve H, Bockelmann W, Franz CM. The 70-kDa FITC dextran reached the jejunum after 15 min and passed the entire small intestine within 1 h after its administration, demonstrating that 4-kDa FITC dextran measured in plasma 1 h after its oral application is a marker of small intestinal permeability. AATEX. Journal Non-Newtonian Fluid Mech., 97, 295-301. 2008;26(11):12618. Vemulapalli V, Bai Y, Kalluri H, Herwadkar A, Kim H, Davis SP, Friden PM, Banga AK. The human skins were from the back or thigh of a 24-year-old woman. European Polymer Journal, 36, 2143-2146. 2011;16(2):518. Because of the recent animal protection rules which have restricted animal experiments [19], we used human cadaver skin as an alternative to animals. The explanation for the anomalous values of a and kM-H is because the mixture solute-solvent is highly compatible due a great compaction of biopolymer, from undisturbed state in the absence of interactions, where free energy of mixing less than zero, and emphasize the nature non-linear or hyperbranched of dextran for Mw high to 200kDa. Charge: Anionic. ; Ortega, A.; Garcia de la Torre, J.; Harding, S.E. Ann Surg Oncol. The calculation of Mark-Houwink parameters is carried out by the graphic representation of the following equation: where kMH (cm3/g) and a are Mark-Houwink constants, depending on the type of polymer, solvent, and temperature of intrinsic viscosity determinations [46]. In the future, we plan to investigate the possibility of enhancing the delivery of other macromolecular drugs such as peptides and siRNAs using the RF-based microporation device. TEWL measurements were performed before and 30min after microporation. Immediately after 2, 5, and 10 times microporations, 97.1432.39, 80.5715.51, and 82.3916.49m sizes of micropores were formed on the skin, respectively. Chromatographic characterization of polymers: Hyphenated and multidimensional techniques. Accueil; Solution; Tarif; PRO; Mon compte; France; Accueil; Solution; Tarif; PRO; Mon compte Ed., 9(2), 85-90. It is worth noting that the size of macromolecules is much bigger than that of solvent (water) molecules [50]. Characterization of high molecular weight dextran produced by Weissella cibaria CMGDEX3. Learn More. (1969). 2018 Jan;184(1):1-11. doi: 10.1007/s12010-017-2522-4. Carbohydrate Polymers 99, 199-207. dextran hydrodynamic 2003;10:33847. One day before the experiment, the skin was stabilized in the skin culture medium. WebThe micelles produced at pH 4.6 have a spherical shape and their size is dependent on the Maillard reaction: reaction time, molar ratio of casein to dextran, and molecular weight of dextran used. A solvent at this temperature is called a theta solvent [53]. This study demonstrated that hydrodynamic volume and radius of gyration of a dextran sample with M w < 20 kDa and its linear counterpart with equal M w are almost Singh TRR, Garland MJ, Cassidy CM, Migalska K, Demir YK, Ryan SAE, Woolfson D, Donnelly RF. Teixeira CG, Fusieger A, Milio GL, Martins E, Drider D, Nero LA, de Carvalho AF. The most widely applied coatings for FDA-approved SPIONs are dextran and carboxydextran. Quantitative data are presented as the meanstandard deviation, and comparisons were carried out using one-way ANOVA (Systat Software Inc., Chicago, IL, USA). The https:// ensures that you are connecting to the Interaction Parameter (); Expansion Factor (); Steric Hindrance Factor (); and Shielding Function F(); for the System PEAOrganic Solvents by Intrinsic Viscosity Measurements. WebFluorescent and biotinylated dextrans are routinely employed to trace neuronal projections. Carbohydrate Polymers, 74, 372-378. CAS After 24h the micropores were either closed or reduced further to smaller sizes, 37.5020.41, 50.5718.07, and 58.5814.99m, for 2, 5, and 10 times microporation, respectively. European Polymer Journal, 43, 1744-1753. (2008). Nair SS. Curvale, R.; Masuelli, M.; Perez Padilla, A. Keywords: FITC dextran; intestinal permeability; transit time. Terms and Conditions, All authors read and approved the final manuscript. WebLabeled dextrans are hydrophilic polysaccharides most commonly used in microscopy studies to monitor cell division, track the movement of live cells, and to report the Rotureau, E.; Dellacherie, E.; Durand, A. The micropores were developed on pig skin and human cadaver skin with dermal and epidermal layers by the microporation device. Boudry I, Blanck O, Cruz C, Blanck M, Vallet V, Bazire A, Capt A, Josse D, Lallement G. Percutaneous penetration and absorption of parathion using human and pig skin models in vitro and human skin grafted onto nude mouse skin model in vivo. This method utilizes promising strategies that have made a significant impact in transdermal delivery [6], they include: iontophoresis, microneedles, sonophoresis, radiofrequency (RF), and chemical enhancers [7,8,9,10,11]. Privacy Lim SB, Tingirikari JMR, Seo JS, Li L, Shim S, Seo JH, Han NS. Ghimici, Luminita; Nichifor, Marieta; Wolf, Bernhard (2009). Senti, F. R.; Hellman, N. N.; Ludwig, G.; Babcock, E.; Tobin, R.; Class, C. A., (1955). Liu Zonghua; Yanpeng Jiao; Yifei Wang; Changren Zhou; Ziyong Zhang (2008). Hydrodynamic Although, intrinsic viscosity is a molecular parameter that can be interpreted in terms of molecular conformation, it does not offer as high resolution on molecular structure as other methods such as Light Scattering, Circular Dichroism, Sedimentation Velocity, Sedimentation Equilibrium, Size Exclusion Chromatography Coupled to Multi-Angle Laser Light Scattering (SEC-MALLS), High-performance Size Exclusion Chromatography (HPSEC), Gel Permeation Chromatography (GPC), NMR, X-rays, etc. Webdextran fraction and the final product depend on changes in the hydrodynamic volume of the molecules after substitution. 10% neutral buffered formalin was purchased from Hisko (Gunpo, Korea). Sci. Nanoparticles in magnetic resonance imaging: from simple to dual WebTry out PMC Labs and tell us what you think. Hydrodynamic American Journal of Food Science and Technology 2013, 1(3), 60-66. Challenges in analysis of high-molar mass dextrans: Comparison of HPSEC, AsFlFFF and DOSY NMR spectroscopy. Synthesis of water-soluble and water-insoluble amphiphilic derivatives of dextran in organic medium. Manage cookies/Do not sell my data we use in the preference centre. Polymers | Free Full-Text | Confinement-Induced Fractionation and Markierte Dextrane sind hydrophile Polysaccharide, die hufig in mikroskopischen Studien zur berwachung der Zellteilung, zum Verfolgen der Bewegung von lebenden Zellen und zur Untersuchung der hydrodynamischen Eigenschaften der zytoplasmatischen Matrix verwendet werden. The range of temperatures used was 25-50 (0.1) C and it was regulated by thermostatic bath Haake C. The viscosity measurements required Hagenbach-Couette correction time (tg 37.31s). J. Braz. Multiple Linear Least-Squares Fits with a Common Intercept: Determination of the Intrinsic Viscosity of Macromolecules in Solution. CAS The study of biopolymers as dextran in aqueous solution, is effectively determined by intrinsic viscosity [] measurements at different temperatures. Article This universalization of the parameters leads to a higher error ratio in the calculation of the respective molecular weights. These constants can be determined experimentally by measuring the intrinsic viscosity of several polymer samples for which the molecular weight has been determined by an independent method (i.e. In addition, we evaluated the regeneration of SC after microporation. The important role of thermodynamic interaction parameter in the determination of theta temperature, dextran/water system. Mark-Houwink-Kuhn-Sakurada Exponent at the Condition. The values of Mark-Houwink parameters universalized for the range of working temperatures are the following: 0.4267 for a*, and 0.1455 cm3/g for k*M-H, with 2 of 0.9399. Journal of Physical Chemistry. Microporation was performed 2, 5, and 10 times on each sample. Drug deliv. The first term on the left-hand side of equation 2 (i/c) has a linear relation as a function of the concentration (c in g/cm3), where is the intrinsic viscosity [] is given by (c0) [42, 43]. 2005;304(1):27486. 2018 Aug 9;23(8):1984. doi: 10.3390/molecules23081984. For a given polymer-solvent pair, the theta condition is satisfied at a certain temperature, called the theta () temperature or theta point. There is a deviation from linearity after 580kDa [69, 70]. Castellanos Gil, E. E.; Iraizoz Colarte, A.; El Ghzaoui, A.; Durand, D.; Delarbre, J.L. These vibrations heat the skin tissue, triggering water vapor and cell ablation, forming microchannels from the SC through to the outer dermis. Nordmeier, Eckliard (1993). 2.It can be found from the figure that at the start-up stage of the shaft, the hydrodynamic effect (HE) is weak, so the HP is not enough to support the shaft, which Experimental and numerical study on the mixed lubrication Advances in Physical Chemistry, Vol. Epub 2012 Oct 18. Carbohydrate Polymers, 38, 267-271. Prausnitz MR, Langer R. Transdermal drug delivery. The numerical value of a indicates that dextrans acquire the shape of a rigid sphere in aqueous solution; and kM-H demonstrates that under water the value decreases with temperature [71, 72]. 2008;28(5):64557. ; Veldhuis, T.F.J. Tirtaatmadja, V.; Dunstan, D. E.; Boger, D. V. (2001). If plotted for all molecular weights, the double logarithm of [] and Mw, the value of a obtained in all cases shows a value smaller than 0.5, which reports that dextran macromolecule has a degree of hyperbranched with increasing molecular weight and increases much for higher Mw (< 200kDa) which making the application of M-H method more difficult. Advanced Drug Delivery Reviews, 60, 1650-1662. Dextrans in Aqueous Solution. Experimental Review on Intrinsic Transdermal delivery of FITC-Dextrans with different molecular weights using radiofrequency microporation, https://doi.org/10.1186/s40824-020-00201-7, http://creativecommons.org/licenses/by/4.0/, http://creativecommons.org/publicdomain/zero/1.0/. Catiker, E.; Guner, A. Bahary, W.S. par | Juil 2, 2022 | mitchell wesley carlson charged | justin strauss net worth | Juil 2, 2022 | mitchell wesley carlson charged | justin strauss net worth By studying the molecular weights of various solutions of polymer, Solomon-Ciuta arrived at the formula which allowed the calculation of the intrinsic viscosity of polymer solutions by a single viscosity determination. WebAmines per 70KDa dextran Theeffectofchargerepulsiononthe hydrodynamicradiuscanbeseenwithRh increaseswithchargedensity.Charge repulsioncausesthehydrodynamicradius toincrease.Withincreasingcharge,the dextranprogressivelyunfolded.Evena lowlevelofaminationcausesthepolymer tounfold. Bookshelf Eremeeva, T. E.; Bykova, T. O. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. Human skins (Full-thickness human skin) were supplied by HansBiomed. The relative browning for dextran from W. cibaria JAG8 was similar to commercial prebiotic Nutraflora P-95 and 3-fold lower than Raftilose P-95. Mark-Houwink values confirm that for these conditions dextrans behave as rigid spheres with a tendency at compaction as temperature increases. Middelkoop E, Bogaerdt AJ, Lamme EN, Hoekstra MJ, Brandsma K, Ulrich MMW. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data. Data File Dextran. Ubbelohde, Leo. What is dextran? Application areas - TdB Labs AB Springer Editions. 2018;1:10915. HHS Vulnerability Disclosure, Help Journal of Applied Polymer Science, 27, 795-810. Advances in chemistry series Washington, DC: American Chemical Society., pp. In a good solvent, which shows a zero or negative heat of mixing with the polymer, the molecule is less-extended, and the intrinsic viscosity is high. The dextran was chosen as another branched polysaccharide which, like the others was suspected to show hyperbranched behavior. 4K, b M.W. The results are shown to be in good agreement with available hydrodynamic models for spherical solutes in cylindrical pores with the effective solute to pore size ratio evaluated using a partitioning model which implicitly WebThomas Gutberlet works at Jlich Centre for Neutron Science (JCNS-2), Forsch and is well known for Lipoteichoic Acid, Neutron Reflectometry and Neutron Reflectivity. The Mark-Houwink a constant is close to 0.75 (or higher) for these good solvents. Determination of intrinsic viscosities of macromolecules and nanoparticles. Bohdanecky, Miloslav (1996). Aqueous solutions of amphiphilic polysaccharides: Concentration and temperature effect on viscosity. Viswanath, D.S. 2013, Article ID 360239, pp. Ioan, C.E. Transdermal drug delivery is an attractive alternative that addresses the limitations of oral and parenteral routes of drug administration; it enables controlled release and long-term systemic drug delivery through the skin, avoids hepatic first-pass effect, avoids gastrointestinal drug degradation, reduces discomfort and trauma resulting from hypodermic injections, and prevents safety hazards associated with dangerous medical waste from needles [1, 2]. 2012;27(3):20212. Journal of Physical Chemistry, 58(11), 953-957. Pore closure examination was performed using an optical microscope (BX43, Olympus, Japan) immediately (time 0), 4, 8, and 24h following device application. Takara, Andres; Acosta, Adolfo; Masuelli, Martin A. Journal of Applied Polymer Science, 72, 871-876. WebTry out PMC Labs and tell us what you think. Dextrans can function efficiently as anterograde or retrograde tracers, The Mark-Houwink parameters is calculated molecular weights range from 8.8 to 200kDa. The receptor phase was collected immediately (time 0) and 2, 4, 8, 12, and 24h. This receptor phase solution was transferred to a 96-well plate and the skin-permeated FITC-dextran was measured using a microplate reader (Molecular Devices, Co. Ltd., Sunnyvale, CA, USA) at 405nm. Table 2 compares the properties of nanoparticles coated with one or other polymer. Chem. These hydrodynamic parameters show that these polysaccharides behave as a compact rigid sphere and contract by the increase of temperature (RH decreases) for the Mw range from 8.8 to 200kDa. Pharmaceutical Quality Dextran 40 is available from Pharmacosmos as active pharmaceutical ingredients (API) as excipient for final formulation, drug product, for medical devices, for production of solutions for injection and infusion and much more. The above data are not useful because it can not be compared with those obtained in this work. Journal of Polymer Science Part B: Polymer Physics, 38, 2591-2598. ; Dutt, N.V.K. Studies carried out at different ionic strength you can see them in the references [64, 65] and works with different solvents in the references [66, 67]. dextran hydrodynamic radius The solutions were prepared in bidestilated water at 1, 0.75, 0.5, 0.25 % wt. For Research Use Only. dextran hydrodynamic radius Mainaa, Ndegwa Henry; Pitknen, Leena; Heikkinen, Sami; Tuomainen; Pivi; Virkki, Liisa; Tenkanen, Maija (2014). The proportionality between []0 and M0.5 is obtained as a limiting law for the no draining regime. Conversely, fluorescein sodium was the compound selected to model hydrophilic, low MW drugs. The Viscosity of Dilute Solutions of Long-Chain Molecules. Challenges for the modern analytical ultracentrifuge analysis of polysaccharides. 2006;3(3):37385. Intrinsic viscosity-molecular weight relationship for chitosan. 4K and M.W. The permeability of fluorescein isothiocyanate-dextrans (FITC-dextrans) with different molecular weights through the pig and human cadaver skins were measured using Franz diffusion cell. Banga AK. An official website of the United States government. Pure Appl. A few percent of R (13) glucosidic linkages provides side chains which appear to be short. Front Microbiol. Part of All authors have consented to the submission of this manuscript for publication. (1987). The molecular mass of dextran as determined by the gel filtration and copper bicinchoninate method was approximately, 800 kDa. A single frequency laser is directed to the sample contained in a cuvette. TEWL measurements were taken 30min after microporation of the human and pig skin. Int J Pharm Investig. Granath, K. A. 8580 Macromolecules 34, Notes - NIST Carbohydrate Polymers 88 1440-1444. Static and Dynamic Light-Scattering Solution Behavior of Pullulan and Dextran in Comparison. Micropores are produced by the arrangement of microelectrodes on the skin at precise dimensions, then RF energy generated by alternating currents induces ionic vibrations between electrodes with positive or negative charges. We believe that the microporation device can be used as a potential technique for effective transdermal drug delivery. For 40 kDa molecules, the hydrodynamic diameter d h ~8 nm is much smaller than the collagen mesh size [33,41]. Advances in Cosmetic Surgery. He, L.; Niemeyer, B. Working with the molecular weights given by the manufacturer and compare them with the molecular weights, calculated from the Mark-Houwink parameters in this work; can be seen that the %RE obtained, see Table 6. Surface morphology study of dextran by scanning electron microscopy showed the porous web like structure. WebHydrodyamic Radius, R H: In addition to the analytic size of a linear (C=1) chain, the end to end distance, R eted or R 0, and the structural size, the radius or gyration, R g, dynamic measurements yield a size called the hydrodynamic radius, R H. Consider a rod particle of length L=10R where R is the radius. FITC-derivatives have been used for a variety of biological applications for more than 40 yeras. Analytical Chemistry. Carbohydrate Research, 340, 811-826. Disclaimer. For a rigid or rod like polymer molecule that is greatly extended in solution, the Mark-Houwink a constant approaches to a value of 2.0 [48].The hydrodynamic radius (RH), for a sphere ((a/b) = 2.5) is given by the Einstein relation [49]. Citation-(RIS Hydrodynamic properties, such as the intrinsic viscosity, [], and equilibrium solution properties such as the hydrodynamic radius RH can be combined to construct dimensionless quantities that are universal since they are of being independent of the macromolecular particle size, while they depend more or less sensitively on its shape [51]. (2007). (2001). HSE and DK helped to porate the skin and to measure TEWL. PMC Hennink, W.E. sharing sensitive information, make sure youre on a federal Furthermore, the influence of temperature on the intrinsic viscosity is given by the chain flexibility parameter (dln[]/dT), which gives information about the conformation of the macromolecule chain in solution [62].
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